Hyaluronic Acid Testing for Cosmetics & OTC Products

Reviewed and Approved by Darren Converse, Certified Laboratories Chemist.

The Complexity of Hyaluronic Acid Testing for Cosmetic & OTC Products

In the cosmetic and skincare industry, few substances have garnered as much attention and acclaim as hyaluronic acid (HA). In fact, the market for hyaluronic acid products is valued at more than $2.6 billion and is projected to grow at a CAGR of 7.46%.

Revered for its remarkable ability to hydrate and plump skin, hyaluronic acid has become a cornerstone in skincare formulations and topical treatments alike.

Behind its seemingly effortless effectiveness lies a complex process of laboratory testing and validation that demands meticulous care and expertise. We explain hyaluronic acid laboratory testing in this article.

Contact us if you need a hyaluronic acid testing laboratory for your samples.

Hyaluronic Acid Laboratory Testing Unveiled

Hyaluronic acid testing isn't merely a task; it's a meticulous journey that requires patience and precision. Here’s a glimpse into the specialized testing regimen that ensures the purity and potency of this coveted substance:

1. Method Development and Validation

Developing a testing method for hyaluronic acid begins with understanding its properties and behavior. Due to its unique ability to absorb water, the testing process must account for potential fluctuations in moisture content. A typical method may involve:

• Three-Day Method Run: This extensive timeframe allows for thorough testing to capture accurate results despite HA’s water-absorbing nature.

2. Moisture Quantification

Moisture content is a critical parameter in HA testing, often specified by customers. Contamination or improper handling can skew results significantly. Key steps in moisture analysis include:

• Desiccator Environment: Samples are kept in a controlled, water-free environment to prevent moisture absorption that could alter results.
• Drying and Weighing: Samples undergo meticulous drying and subsequent weighing to precisely quantify moisture levels.

3. Chemical Analysis

Hyaluronic acid purity is verified through chemical analysis, typically using High-Performance Liquid Chromatography (HPLC). This technique ensures accurate quantification of HA content in raw materials and finished products.

4. Stability and Performance Testing of Hyaluronic Acid

Beyond purity, HA’s performance over time is evaluated through stability testing. This involves:

• Hydration and Swelling Tests: Samples are subjected to controlled conditions to observe swelling behavior over hours or days, crucial for understanding product performance and shelf-life.

5. Certificate of Analysis

Upon completing testing and analysis, a comprehensive Certificate of Analysis (COA) is issued. This document provides clients with assurance regarding the quality, purity, and performance characteristics of the cosmetic or OTC product that includes hyaluronic acid.

The Art and Science of Hyaluronic Acid Testing

Laboratory testing of products formulated with hyaluronic acid is a blend of scientific rigor and meticulous attention to detail. Each step, from method development to final COA issuance, plays a crucial role in ensuring that the product meets the highest standards of purity and efficacy. It's through these exacting processes that HA continues to uphold its reputation as a transformative ingredient in skincare and beyond.

Whether you're a skincare enthusiast or a scientist in the field, appreciating the journey of hyaluronic acid, from testing to application, enhances your understanding of its impact on health and beauty.

Contact us if you need hyaluronic acid laboratory testing for your samples.